Journal: Cell Reports Medicine
Article Title: Single-cell profiles delineate immune cell remodeling and enhanced tumor-fibroblast interaction of hepatoblastoma after chemotherapy
doi: 10.1016/j.xcrm.2025.102401
Figure Lengend Snippet: Identification of FGF-FGFR signaling associated with tumor cell proliferation and anti-tumor efficacy evaluation (A) Sub-clusters of stomal cells (upper) and FGFs scores (lower). (B) Ligand-receptor interactions between tumor cells and CAF_c3_MMP11 + in primary and post-chemotherapy samples. (C) Expression of FGF family ligands in stromal components in primary and post-chemotherapy samples. (D) Overall expression of FGF family ligands in CAF_c3_MMP11 + cells in primary and post-chemotherapy samples (∗∗∗ p < 0.001). (E) Colocalization of CAF signatures, hepatic progenitor signatures, and FGF ligands and receptors and spatial distance of CAF_c3_MMP11 + and HECs. Spatial cell-cell interaction of FGF9-FGFR4 and FGF3-FGFR4 compared with FGF9-FGFR1 and FGF3-FGFR1 were exhibited. (F) Scheme of patient-derived samples used to establish the HB PDX model and subsequent pharmacological intervention in vehicle, PD173074 and roblitinib groups. (G) In vivo tumorigenesis assays by vehicle or two TKIs treatment in PDX models. Scale bars: 1 cm. (H) and (I). In vivo tumorigenesis assays by vehicle or two TKIs ( PD173074 , roblitinib) treatment in two subcutaneous PDX models. Data are presented as mean tumor volume ±95% CI. (∗ p < 0.05 and ∗∗∗ p < 0.001). (J) mIHC images of co-expression of AFP (green), KI67 (red), and DAPI (blue) in section of PDX tumor tissues from control and roblitinib-treated groups, collected at endpoint. Scale bars: 50 μm. (K) The abundance of KI67 + AFP + tumor cells in control and roblitinib-treated tumors (∗ p < 0.05).
Article Snippet: FGFR4 , Proteintech , 11098-1-ap; RRID: AB_2103657.
Techniques: Expressing, Derivative Assay, In Vivo, Control
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